Fluorometric quantitation of broth-cultured mycoplasmas by using alkaline ethidium bromide
نویسندگان
چکیده
منابع مشابه
Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli
BACKGROUND Efflux pump activity has been associated with multidrug resistance phenotypes in bacteria, compromising the effectiveness of antimicrobial therapy. The development of methods for the early detection and quantification of drug transport across the bacterial cell wall is a tool essential to understand and overcome this type of drug resistance mechanism. This approach was developed to s...
متن کاملQuantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide
Characterization of an aqueous extract of human placenta, used as a licensed drug for wound healing, leads to the identification of several bioactive components including polydeoxyribonucleotides (PDRNs). PDRNs are mixture of DNA fragments of different molecular weight. A spectrofluorimetric method of quantitation of PDRNs in the aqueous extract of human placenta by using ethidium bromide (EtBr...
متن کاملFluorometric Quantitation of dsDNA Using PicoGreen®
An essential element of cellular and molecular biology is the ability to quantitate DNA in large numbers of samples at a sensitivity that enables determination of small amounts of sample. Here we describe a method to quantitate dsDNA using the BioTek SynergyTM HT and SynergyTM 2 Multi-Detection Microplate Readers.
متن کاملFluorometric Quantitation of dsDNA
Introduction Many techniques of cellular and molecular biology require the ability to quantitate dsDNA in large numbers of samples at sensitivities that only require a small amount of the total sample. Isolation of plasmids from bacterial cultures, genomic DNA from mammalian cells, cDNA synthesis for library production, and quantitation of PCR products for diagnostic purposes all require the di...
متن کاملRapid quantitation of mRNA species in ethidium bromide-stained gels of competitive RT-PCR products.
A rapid method for quantifying the low-abundant mRNAs of the low density lipoprotein receptor and the 3-hydroxy-3-methylglutaryl coenzyme A reductase by competitive polymerase chain reaction is presented. This approach requires neither special labeling nor blotting procedures. For each analysis, a defined amount of total cellular RNA is co-reverse transcribed and co-amplified with a titration s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1993
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.31.5.1303-1307.1993